Morpholino zearalins

ABSTRACT

Resorcyclic acid lactone compounds of the formula   WHEREIN -A- is either -CH2-CH2- or -CH CH-, and   IS EITHER   AND THEIR DERIVATIVES ARE PROVIDED FOR ANABOLIC AND ESTROGENIC ACTIVITY AND AS RUMINANT GROWTH PROMOTERS.

i United States Patent 1191 Hodge MORPHOLINO ZEARALINS {75] Inventor: Edward B. Hodge, Terre I-Iaute, Ind.

[73] Assignee: Commercial Solvents Corporation,

Terre Haute, Ind.

[22] Filed: Jan. 21, 1974 [21] Appl. No.: 435,294

[52] US. Cl. 260/247.2 B; 424/248 [51] Int. Cl C07d 87/36 [58] Field of Search 260/2472 B [56] References Cited OTHER PUBLICATIONS W. H. Urry, Tetrahedron Letters, 3109-3114.

Vanden Heuvel, W. J. A., Separ. Sci., 1968, 3(2) Primary Examiner--Robert Gerstl Assistant ExaminerPaul J. Killos Attorney, Agent, or Firm-Morton, Bernard, Brown, Roberts and Sutherland 1 1 ABSTRACT Resorcyclic acid lactone compounds of the formula Oct. 28, 1975 OH O CH II I C-O-CH- (CI-1 wherein -A- is either CH C1-I or --CH=CI-I-, and

is either and their derivatives are provided for anabolic and estrogenic activity and as ruminant growth promoters.

5 Claims, No Drawings MORPHOLINO ZEARALINS This invention relates to novel compounds having anabolic and estrogenic properties. More particularly, it relates to compounds that can be prepared by reacting morpholine and formaldehyde with various resorcylic acid lactone derivatives.

The compounds of the present invention, hereinafter referred to as the M compounds, are those of the formula:

OH O CH 1. l. 3 o O H (Cll )3\ Z no A (CH )3 H CH 2 2 wherein A- is either CH -CH or CH=CH, and

is either CH c=o or CH-OH.

The hydrogen of the hydroxyl groups in the second and fourth positions of the resorcyclic acid lactone part of the molecule can be substituted, for instance with substituted and unsubstituted alkyl, e.g., containing from 1 to about 15 carbon atoms including lower alkyl such as methyl, ethyl, hexyl, etc., and cycloalkyl, particularly monocyclic cycloalkyl of about 5 to 8 carbon atoms, such as cyclopentyl, cyclohexyl, methyl cyclohexyl, etc.; alkanoyl, generally containing 1 to about 25 or more carbon atoms including lower alkanoyl such as acetyl, propionyl, valeryl, etc.; substituted or unsubstituted aryl, for instance, monocyclic aryl containing about 6 to 10 carbon atoms or more, such as phenyl, toly], etc.; and aryl alkyl (that is an alkyl group having an aryl substituent thereon), wherein the aryl substituent may be monocyclic aryl containing about 6 to 10 carbon atoms or more and the alkyl group is generally lower alkyl, for example, 1 to about 6 carbon atoms,

examples of such aryl alkyl compounds including benwherein A, and

are as defined above.

The reaction of the morpholine, or a derivative of it, formaldehyde, and zearalin derivative to prepare the M compounds can generally be effected at about 25 to 150C., preferably about 60 to C., and preferably the reactants are in solution in solvent-providing amounts at an inert solvent such as isopropanol, ethan01, methanol, or alkyl ethers. The solvent is frequently provided in an amount of 1 to 100 or more, particularly 3 to 50, times by weight, of the zearalin. Reaction time will vary depending upon the particular zearalin employed, temperatures used, etc., but the reaction will often be complete in about 3 hours or less, for instance, about 0.1 to 2.5 hours. The ratios of the reactants to be employed in the reaction mixture are generally about 1 to 5 moles of formaldehyde and about 1 to 5 moles of morpholine, per mole of the zearalin. Preferably, the molar ratio of formaldehyde to morpholine used will be in the range of about 1:1 to about 2:1.

Recovery and refinement of the compounds of the present invention can be by conventional techniques, for example by crystallization, filtration, and recrystallization.

The Zearalins employed making the M compounds can be prepared by known methods. Zearalins can be prepared, for instance, from zearalenone (A- is CH=CH-, and

which can be obtained by cultivation of the microorganism Gibberella zeae (Gordon) in a suitable fermentation medium, as described, for example, in US. Pat. No. 3,196,019.

Zearalenone may conveniently be employed to prepare others of the Zearalins which may be used to pro vide the M compounds. For instance, the unsaturated carbon bond in the lactone zearalenone ring can be hydrogenated according to the procedure of US. Pat. No. 3,239,354. The keto group of zearalenone may be converted to CHOH by the procedure disclosed in U.S. Pat. No. 3,239,341.

The keto group of zearalenone may be connected to by a procedure also disclosed in U.S. Pat. No. 3,237,341. Replacement of the hydrogen of the hydroxy groups of the zearalins with an alkyl, alkanoyl, aryl, or aryl alkyl radical is disclosed in U.S. Pat. Nos. 3,239,342, 3,239,347, and the above-mentioned patents.

Zearalenone, obtained by fermentation, as in U.S. Pat. No. 3,196,019, is in the trans-configuration. Transzearalenone, or zearalins prepared therefrom, wherein A is -Cl-l=Cl-l-, may be converted to the corresponding cis configuration by electromagnetic irradiation of the trans isomer in the 2,800-3,500 angstrom wave length range, as disclosed in U.S. Pat. Application Ser. No. 317,117, filed Dec. 21, 1972, now abandoned.

Zearalenone, obtained by fermentation as in U.S. Pat. No. 3,196,019, is an S conformer. The R conformer of zearalanone may be obtained from S- zearalenone by the procedure of U.S. Pat. Application Ser. No. 452,260, filed Mar. 18, 1974.

Zearalins, wherein I z is CHOH,

can, anddo, exist in two different diastereoisomeric arrangements based on the substitutions on the carbon atom. Diastereoisomers, or diamers as they are commonly-called, are stereoisomers which are not enantiomorphs (i.e., are not mirror images). These diamers have different melting points and, for that reaadministered in an amount of about 1 to 200, preferably l to 50 milligrams per head per day.

.The M compound can be administered in combination with a pharmaceutically-acceptable carrier. For example, the M compound can be employed as an additive in their feed or as an implant under the skin. For example, the compounds can be blended with ordinary feed which contains nutritional values in an amount sufiicient to produce the desired rate of growth, or the compounds can be suspended in a suitable injection suspension medium, such as peanut oil, and injected parenterally. From 2.5 to 50 grams of the compound per ton of feed is typical. When an implant is used, for example a ball or cylindrical implant inserted under the skin on theear of an animal, the implant will generally contain from 1 mg. to 100 mg. of the compound. Other modes of parenteral administration include intramuscular, intravenous, and intraperitoneal injections.

When an M compound is to be administered to ruminants in their feed, a feed composition may be prepared containing the usual nutritionally balanced quantities of carbohydrates, proteins, vitamins, and minerals, together with the compound. Some of the usual sources of these dietary elements are grains, such as ground grain and grain by-products; animal protein substances, such as those found in fish meal and meat scraps; vegetable proteins, such as soybean oil meal or peanut oil meal; vitaminaceous materials, e.g., mixture of vitamins A and D, riboflavin supplements and other vi" min B complex members; and bone meal and limestoir to provide minerals. A type of conventional feed mate rial for use'with cattle, for example, includes alfalfa-ha andground corn'cobs, together with supplementary vitaminaceous substances if desired. r

The following examples are offered to illustrate this invention; however, the invention is not limited to the specific materials, amounts, and procedures set forth.

EXAMPLE I This example illustrates the preparation of 5- morpholinomethyl zearalerione, i.e., the compound of the formula:

A mixture of 50 cc. of isopropanol, 3.8 g. of transzearalenone, 1.0 ml. of a 37 weight percent aqueous solution of formaldehyde, and 1.0 ml. of morpholine are reacted for 2 hours on a steam bath. The resultant solution is then cooled for 3 hours in a refrigerator, whereupon 3.8 grams of trans-5-morpholinomethyl zearalenone crystallizes out of the solution. The crystals are separated from their mother liquor by filtration and are observed to have a melting point of l62l63C.

- EXAMPLE ll This example illustrates the preparation of 5- morpholinomethyl zearalanol, i.e. the compound of the formula:

A mixture of 50 cc. of isopropanol, 3.8 g. of the high melting diamer of zearalanol, 1.0 ml. of a 37 weight percent aqueous solution of formaldehyde, and 1.0 ml. of morpholine are reacted for 2 hours on a steam bath. The resultant solution is then cooled for 3 hours in a refrigerator, whereupon 3.8 grams of 5- morpholinomethyl zeralanol crystallizes out of the solution. The crystals are separated from their mother liquor by filtration and are observed to have a melting point of 164-l69C. Recrystallization from ml. of isopropanol gives 1.2 grams of 5-morpholinomethyl zearalanol having a melting point of l65167C.

EXAMPLE III This example illustrates the estrogenic activities of 5-morpholinomethyl zearalenone and 5- morpholinomethyl zearalanol.

Samples of trans-S-rhorpholinomethyl zearalenone and 5-morpholinomethyl zearalanol are tested for uterotropic activity according to the well known mouse uterine test. This test consists of feeding the test material in admixture with a standard feed, to eight, adult, ovariectomized female mice at a ration of 3 grams per day for a 5 day period. On day six, the animals are weighed and sacrificed, and their uteri removed and weighed. Estrogenic activity is confirmed if the uterus of the test mouse is heavier and accounts for a greater percentage of the mouses body weight than the uterus of a control mouse. Test results are reported in Table I.

morpholinomethyl zearalenone as a growth promoting supplement in animal feed.

For young beef cattle, i.e., calves to yearlings running to 2 year olds, each animal is given 5 to milligrams per day of trans-S-morpholinomethyl zearalenone intimately admixed in about 18 to 22 pounds per head per day of a complete pelleted ration for about 180 days. The complete pelleted ration includes, in addition to trans-S-morpholinomethyl zearalenone, the following:

Barley, weight percent 40-43 Molasses dried beet pulp, 34.5-37.5 Alfalfa pellets, 8.0 Tallow, 2.5 Calcium carbonate, .30 Urea, .30 Phosphorus source, .40 Salt, .50 Molasses, 10.00 Trace mineral, 0.5 Vitamin A, MMl.U/ton 24 Note: Milo or corn. for example, can be substituted for the barley.

The trans-5-morpholinomethyl zearalenone is admixed with the above ingredients in a stationary blender or a feed mix truck in the following amounts in grams per ton to provide an appropriate complete pelleted feed with dosage levels ranging from 5 to milligrams per head per day.

Grams/ton: Mg/head/day These gram amounts are premixed with, for example, 10 pounds of soybean hulls prior to admixture with the other ingredients.

EXAMPLE V This example illustrates the use of 5- morpholinomethyl zearalanol as a growth promoting supplement in animal feed.

For young swine, i.e., 6 week old pigs to about pound pigs, each animal is given 5 to 2 milligrams per day of S-morpholinomethyl zearalanol intimately admixed in about 1 /2 to 5 /2 pounds per head per day of a grower ration until it reaches a weight of about 100 pounds. When the swine weigh between 90 and pounds, the feed is changed to one whereby each animal is given 20 to 50 milligrams per day of the compound intimately admixed in about 5 /2 to 10 pounds per head per day of -a finisher ration until it reaches market weight of about 220 pounds. The grower and finisher ration include, in addition to the 5- morpholinomethyl zearalanol, the following:

The S-morpholinomethyl zearalanol is admixed with the above ingredients in a blender in the following amounts in milligrams per pound to provide an appropriate feed with dosage levels ranging from 6 to 96 milligrams per head per day.

M g/pound: Mg/head/day 2 6 4 l 2 8 24 16 48 3 2 96 EXAMPLES Vl TO IX The procedure of Example I is essentially repeated, except employing different zearalins to provide other M compounds of this invention. The zearalins, which are employed, have the formula:

OH O CH O CH (CH HO A (CH )3 to provide an M compound of the formula OH O CH C O CH (CH HO A (CH2)3 T2 N 1 W H2C\ /CH2 0.

TABLE II VII -CH2-CH2- CH -CH -CH2- 2 CHOH I claim: 1. A compound of the formula:

5 OH i 3 0- o CH (CH2)3 wherein -A is either -CH CH or CH=CH-, and

is either CH2, r CH-OH.

2. The compound of claim 1 wherein --A is CH- CH 3. The compound of claim 2 wherein 2 is CH-Ol-l.

4. The compound of claim 1 wherein A-- is -CH=CH--.

5. The compound of claim 4 wherein 

1. A COMPOUND OF THE FORMULA:
 2. The compound of claim 1 wherein -A- is -CH2-CH2-.
 3. The compound of claim 2 wherein
 4. The compound of claim 1 wherein -A- is -CH CH-.
 5. The compound of claim 4 wherein 